The genetic depletion of the protein target in cells provides an unequivocal approach to test the specificity of antibodies. Contrary to common belief, monoclonal antibodies are not necessarily monospecific. In fact, the study of the natural immune response to retroviruses shows that polyspecificity and cross-reactivity of antibodies are frequently observed (1, 2).
The proof of specificity of diagnostic, research and therapeutic antibodies is therefore critical to ensure your affinity reagents meet high quality standards. We at Emerge Biotech are using our expertise and knowledge to help you achieve your goals by identifying superior antibodies.
(1) Keitel T, Kramer A, Wessner H, Scholz C, Schneider-Mergener J, Hohne W (1997) Cell, 91, 811-20.
(2) Kramer A, Keitel T, Winkler K, Stocklein W, Hohne W, Schneider-Mergener J (1997) Cell, 91, 799-809.